TABLE 1.
Monoclonal antibody panel used and cell types enumerated
| Monoclonal antibodiesa | Cell type enumerated |
|---|---|
| CD45/CD14 | Lymphocytes (% in gating region) |
| IgG1/IgG2 | Isotype-matched control |
| CD3/CD4 | CD4+ T cells |
| CD3/CD8 | CD8+ T cells |
| CD4/HLA-Dr | Activated CD4+ cells |
| CD4/CD45RO | Memory CD4+ cells |
| CD4/CD26 | CD4+ cells reactive against recall antigens |
| CD4/CD28 | CD4+ cells expressing CD28 costimulatory molecule |
| CD8/CD45RO | Memory CD8+ cells |
| CD8/CD26 | CD8+ cells reactive against recall antigens |
| CD8/CD28 | Cytotoxic T-cell precursors |
| CD8/CD38 | Activated CD8+ cells |
| CD8/CD57 | Cytotoxic (MHC-nonrestricted) CD8+ cells |
a
All monoclonal antibodies were manufactured by Becton Dickinson, except CD4/CD26, CD4/CD28, and CD8/CD26 (Coulter). IgG, immunoglobulin G.
b
MHC, major histocompatibility complex.