Figure 2.

Cell-cycle analysis of colorectal adenocarcinoma cells treated with midostaurin (PKC412). Mouse and human colorectal adenocarcinoma cells were treated with midostaurin at 0 (a,d,g), 5 (b,e,h), and 20 μM (c,f,i) for 24 h. After treatment, cells were fixed and stained with propidium iodide. Cell-cycle distribution was acquisitioned by flow cytometry. Representative DNA histograms of colorectal cells treated with PKC412 were shown and the expression percentage of each cell-cycle phase was indicated in the panels. Data from four separate experiments were expressed as mean ± SEM.