Figure 6.

Effect of midostaurin (PKC412) on expression of putative target proteins in CT26 (a), HCT116 (b), and SW480 (c). Cells were treated with PKC412 for 24 h and then cells were lysed and harvested. Equal amounts of proteins were subjected to immunoblotting to detect midostaurin targets and Trex-1 proteins. Representative blots of midostaurin target proteins were shown. The densitometric analysis of each band was shown in the bottom panel. Original Western Blot images can be found in Figure S1.