Figure 8.

Effect of DNA-sensing signaling and molecular target proteins in midostaurin (PKC412)-treated spleen macrophages. Macrophages were treated with PKC412 for 2 or 24 h and then cells were lysed and harvested. RNA samples were isolated followed by reverse transcription of RNA using primers of DNA-sensing specific genes (a). Equal amounts of proteins were subjected to immunoblotting to detect midostaurin targets and Trex-1 proteins. Representative blots of midostaurin target proteins were shown (b). The densitometric analysis of each band was shown in the bottom panel. Data from three mice spleen macrophages were expressed as mean ± SEM. Significant differences between control cells and cells treated with PKC412 are indicated by * p < 0.05 and *** p < 0.001. Original Western Blot images can be found in Figure S1.