TABLE 5.

Quality control serum reproducibility of the ELISA used in this study with sera A143, A144, A146, and A148

Run	Operator	No. of replicates/run	No. of points/run	Within run		Between run		Overall
				%CVa	%CVb	%CVa	%CVb	%CVa	%CVb
A143								25.6	26.1
Series 1	1	6	1			17.1	24.3
1				13.1	19.4
2				23.6	19.9
3				5.1	28.2
Series 2	2	6	1			28.1	26.4
1				13.1	16.3
2				38.5	17.1
3				8.4	10.9
A144								17.3	17.5
Series 1	1	6	1			11.7	12.1
1				7.7	7.3
2				5.7	11
3				2.6	8.4
Series 2	2	6	1			14.7	20.8
1				12.4	18.2
2				11.8	17.3
3				5.7	19.8
A146								13.1	17.2
Series 1	1	6	1			10.2	19.8
1				8.6	19.2
2				10.6	7.8
3				5.0	6.1
Series 2	2	6	1			13.6	12.7
1				5.4	9.4
2				9.4	12.8
3				5.7	15.2
A148								12.3	16.8
Series 1	1	6	1			8.4	18.4
1				3.1	16.3
2				11.1	11
3				4.6	11.9
Series 2	2	6	1			12.3	13.2
1				10.0	14.4
2				14.4	9
3				3.7	8.8

^aAverage of the individual point %CVs calculated by the absorbancy of each point. 

^bAverage of the individual point %CVs calculated by the absorbancy of each inhibited point.