Table 3.

Effect of diet on alpha diversity of gut microbiota in mice

Alpha diversity indices	Groups				Significance (p value)
	CD	CD + A	HFD	HFD + A	CD vs CD + A	CD vs HFD	HFD vs HFD + A
OTUs	6468.7 ± 568.6	4871.6 ± 678.5	6543.4 ± 634.1	5104.7 ± 686.5	0.0013	NS	0.0053
Chao1	8682.7 ± 42.69	7152.3 ± 74.4	9354.4 ± 651.4	7500.5 ± 46.09	< 0.0001	0.0229	0.0002
Shannon	6.99 ± 0.0132	5.63 ± 0.020	8.19 ± 0.055	6.39 ± 0.018	< 0.0001	< 0.0001	< 0.0001
Simpson	0.933 ± 1.110	0.912 ± 0.00	0.979 ± 0.00	0.935 ± 0.00	NS	NS	NS

Values are expressed as the mean ± SD (n = 6). Data were analyzed by One-way ANOVA followed by Bonferroni post-hoc test,  p≤ 0.05, NS =not significant, CD: Standard-chow diet for 3 weeks; CD + A: Standard-chow diet for 3 weeks and subjected to amoxicillin treatment (50 mg/kg body weight) in 3rd week; HFD: High-fat diet for 3 weeks (73% energy from fat); HFD + A: High-fat diet for 3 weeks and subjected to amoxicillin treatment (50 mg/kg body weight) in  3rd week