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. 2022 Oct 6;14(19):4891. doi: 10.3390/cancers14194891

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Multi-assay analysis of the Landscape and CDX2-targeted immunofluorescence (IF) assays analyzed by OCULAR. (A) Statistically significant (p ≤ 0.05) rare-event-count correlations across the two IF assays, with the red-to-blue color gradient indicating a negative-to-positive correlation, respectively. (B) Cluster occupancy of the rare cells identified by the CDX2-targeted and Landscape stains when using the 8-group hierarchical clustering model. (C) t-Stochastic neighbor embedding (t-SNE) plot of the 8 cell clusters comprised of rare cells from both stains, as indicated by the markers’ shape. (D) Representative gallery of the 8 clusters with cells from both assays. Each row represents a cluster with a cell from the CDX2-targeted protocol on the left and a cell from the Landscape protocol on the right. Images taken at 100× magnification. Scale bars represent 100 µm.

Multi-assay analysis of the Landscape and CDX2-targeted immunofluorescence (IF) assays analyzed by OCULAR. (A) Statistically significant (p ≤ 0.05) rare-event-count correlations across the two IF assays, with the red-to-blue color gradient indicating a negative-to-positive correlation, respectively. (B) Cluster occupancy of the rare cells identified by the CDX2-targeted and Landscape stains when using the 8-group hierarchical clustering model. (C) t-Stochastic neighbor embedding (t-SNE) plot of the 8 cell clusters comprised of rare cells from both stains, as indicated by the markers’ shape. (D) Representative gallery of the 8 clusters with cells from both assays. Each row represents a cluster with a cell from the CDX2-targeted protocol on the left and a cell from the Landscape protocol on the right. Images taken at 100× magnification. Scale bars represent 100 µm.