Table 1.

Rare-event frequencies, enumerations and sample positivity from the Landscape and CDX2-targeted immunofluorescence (IF) protocols. The sample positivity threshold of ≥5 events/mL was determined by comparisons to the rare-event enumerations of a randomly selected normal donor cohort. The frequency of each classification is provided as a percentage of the total rare-event profile for each IF protocol.

IF	Event Classification	Sample Positivity	Mean (Events/mL)	Standard Error (±Events/mL)	% of Total Rare Events	Median (Events/mL)	Range (Events/mL)
Protocol
Landscape	DAPI only	15/18	13.69	2.9	3.55	10.84	0.00–51.57
	CK (Epi.CTC)	18-Jul	57	36.36	14.77	3.55	0.00–549.63
	Vim	18-Nov	6.64	1.27	1.72	7.3	0.00–20.16
	CD45/CD31	18-Oct	10.29	2.44	2.67	8.05	0.00–33.16
	CK|Vim (Mes.CTC)	18-Jun	7.6	1.4	1.97	1.4	0.00–91.29
	CK|CD45/CD31	18-Apr	4.66	1.86	1.21	2.13	0.00–30.74
	Vim|CD45/CD31	18-Nov	24.52	8.73	6.35	7.79	0.00–121.00
	CK|Vim|CD45/CD31	15/18	100.4	35.02	26.01	12.89	0.00–453.13
	Onc CK	18-Dec	71.59	38.39	18.55	6.64	1.06–657.60
	Onc CK|Vim	18-Oct	32.72	13.64	8.48	7.38	0.00–217.65
	Onc CK|CD45/CD31	0/18	1.47	0.36	0.38	1.12	0.00–4.47
	Onc CK|Vim|CD45/CD31	14/18	55.37	16.22	14.35	34.32	0.00–268.32
CDX2- targeted	DAPI only	18/18	63.72	12.8	8.33	45.29	16.94–226.94
	CK	18-Dec	88.14	43.95	11.53	11.97	0.00–597.32
	CDX2	18-Dec	11.45	3.28	1.5	7.27	1.04–60.22
	CD45	18-Mar	5.44	3.35	0.71	0	0.00–59.22
	CK|CDX2 (CDX2.CTC)	14/18	19.95	6.71	2.61	11.34	0.00–124.08
	CK|CD45	18-Dec	36.66	13.71	4.79	10.02	0.00–203.63
	CDX2|CD45	18-Dec	29.37	12.46	3.84	9.8	0.00–185.68
	CK|CDX2|CD45	14/18	143.67	101.35	18.79	21.79	1.44–1843.34
	Onc CK	15/18	123.55	60.73	16.16	26	2.31–1035.65
	Onc CK|CDX2	18/18	222.4	65.04	29.08	114.87	15.55–1151.64
	Onc CK|CD45	0/18	0.06	0.06	0.01	0	0.00–1.06
	Onc CK|CDX2|CD45	18-Oct	20.27	7.64	2.65	14.5	0.00–1138.28