Figure 8.

Hemin enhances escaping from senescence in hypoxic cells. In HCT116 cells, the gene encoding CAT was silenced with the use of siRNAs. Cells were cultured in parallel in normoxia or hypoxia. Cells were exposed to 100 μM hemin (□). Control cells were subjected to IRINO and NaOH in the corresponding dose (■). (A) Evaluation of cell number. Cell number was counted with the use of Bürker’s chamber. (B) Evaluation of SA-β-gal positive cells; (C) Activity of SA-β-gal enzyme. Cells were cytospined and cytochemical staining for SA-β-gal activity was performed. Representative photos were acquired using light microscopy. Original magnification 200×, scale bar 50μM; Each bar represents mean ± SEM, n ≥ 3; ** p < 0.01, *** p < 0.001—vs. si-NEG control normoxia; $ p < 0.05—control vs. hemin 100 μM.