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. 2022 Sep 30;14(19):4793. doi: 10.3390/cancers14194793

Figure A3.

Figure A3

Evaluation of protein expression in HCT116 cells treated with 2.5 μM IRINO subjected to 5 or 10 μM H2O2 after the 4th and 7th days of the experiment: cell cycle inhibition and proliferation markers. Quantification is based on the densitometry analysis performed with the use of ImageJ software. Data are shown as the ratio of the protein amount to respective protein loading control (GAPDH): (A) p21; (B) p-S6; (C) p53; (D) cyclin A; (E) cyclin B; (F) p-cdc2; (G) p-Rb. Each bar represents mean ± SEM, n ≥ 3; ** p < 0.01, *** p < 0.001—vs. 1 + 4 control; $ p < 0.05, $$ p < 0.01—1 + 7 vs. 1 + 4.