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View full-text article in PMC

. 2022 Sep 23;14(19):4617. doi: 10.3390/cancers14194617

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Circulating hybrid cells (CHCs) detected with an enhanced antibody cocktail correlate with progression free survival and overall survival. (A) Peripheral blood mononuclear cells stained with antibodies to gp100/HTR2B (white) and CD45 (red) facilitate identification of CHCs and circulating tumor cells (CTCs). CHCs are distinguished from basophils and T regulatory cells (Treg) based upon lack of expression of CD203c and CD25 (green), respectively. Higher magnification of boxed regions with individual antibody staining shown. (B) Enumerated CHCs and CTCs in healthy subjects and patients across stage. Asterisk indicates p < 0.05. (C) Levels of gp100⁺ CHCs and gp100⁺/HTR2B⁺ CHCs from three representative patients from each evaluated stage. (D) CHCs harboring heterogeneous protein expression identified in a class 2 patient. (E,F) High levels of CHCs correlate with progression free survival and overall survival, whereas there is no correlation for CTCs (G,H).