Figure 2.

Specific internalization of DARPin-PNA-SMVs by HER2-overexpressing cells. SK-BR-3 (A) and MDA-MB-231 (B) cells were incubated with 50 nMDARPin-PNA-SMVs, containing the fluorescein-labelled oligonucleotide (Flu-CCCTAA) for 30 min at 37 °C. The cells were washed twice with culturing medium and subsequently incubated at 37 °C for 6 h (see Materials and Methods). Nuclei were stained with Hoechst 33342. Superimposed confocal fluorescent images of the cells in blue-green and blue-red fluorescence channels are presented.