FIG. 3.

Effect of incubation temperature on the percentage of PMN expressing CD11b (A) and mean channel shift (B) in cytokine-treated whole-blood cultures. Heparin and EDTA whole-blood cultures were incubated for 18 h at 37°C with either PBS (UN), IFN-γ, GM-CSF, IFN-γ plus GM-CSF, TNF-β, or IL-6 and then stained for CD11b as described in Materials and Methods. (A) The corresponding time zero controls are indicated by boldface dotted lines. Solid bars, 37°C; stippled bars, RT. (B) Vertical solid and dotted lines drawn through the histograms indicate the mean channel numbers corresponding to the untreated controls at 37°C and RT, respectively. For both panels, results are representative of experiments using two donors.