Figure 1.

Cisplatin induces specific immune effects in bladder cancer cell line. T24 cell lines were treated with 2 μg/mL cisplatin (T24 DDP group) or PBS (T24 PBS group) for 24 h at standard condition. (A) The different genes expression panels between T24 DDP group and T24 PBS group were showed in heatmap. (B) Gene expression levels were quantified as Fragments Per Kilobase Million (FPKM) and then Log₂–transformed as A value. The significant different expression genes were identified as absolute fold change of A value > 1.5 and Padj (adjust p) value < 0.05 between two groups. Finally, the volcano map showed 235 up genes and 129 down genes with significant difference. (C) Go pathways with Padj value < 0.05 were showed here between T24 DDP group and T24 PBS group. (D) Gene set enrichment analysis (GSEA) in: Interferon α, β signaling (p < 0.001), cytokines and inflammatory response (p < 0.001) and cytosolic DNA sensing pathway (p < 0.002). (E) Relative transcription levels of genes expression involved in IRF3 and NF-κB signal pathway or targeting genes in T24 DDP group and T24 PBS group. Gene expressions were normalized by R software from −1.5 to 1.5 (Low to high, blue to red).