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. 2022 Sep 27;11(19):3011. doi: 10.3390/cells11193011

Figure 3.

Figure 3

STING targeting siRNAs and STING specific inhibitor H151 significantly reversed cisplatin related immune effects. (A) Knockdown efficiency validation of STING in T24 and TCCSUP cell lines. GAPDH was used as control protein. (B) T24 and TCCSUP cell lines were treated with non-targeting (Scramble, Scr) or two STING-targeting siRNA (si2 and si3) for 48 h before treatment with 2 μg/mL cisplatin for 0 h, 4 h, 8 h, 12 h, 16 h, 20 h, respectively. IFN-β mRNA and IL-6 mRNA were then analyzed by qRT-PCR. (C) Western blotting results of key proteins involved in cGAS-STING pathway in T24 and TCCSUP cell lines treated with non-targeting (Scramble, Scr) or two STING-targeting siRNA (si2 and si3) for 48 h before treatment with 2 μg/mL cisplatin for 0 h, 8 h, 16 h, 24 h, respectively. GAPDH was used as control protein. (D) T24 and TCCSUP cell lines were co-treated with DMSO or H151 and 2 μg/mL cisplatin for 0 h, 4 h, 8 h, 12 h, 20 h, 28 h, respectively. IFN-β mRNA and IL-6 mRNA were then analyzed by qRT-PCR. (E) Western blotting results of key proteins involved in cGAS-STING pathway T24 and TCCSUP cell lines co-treated with DMSO or H151 and 2 μg/mL cisplatin for 0 h, 8 h, 16 h, 24 h, respectively. GAPDH was used as control protein. * p < 0.05, ** p < 0.01.