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. 2022 Sep 27;11(19):3011. doi: 10.3390/cells11193011

Figure 5.

Figure 5

Cisplatin induced dsDNA and the releasing of chromatin bound cGAS may function as an important role in enhancing STING signal. (A) Basic transcription levels of cGAS in bladder cancer cell line on CCLE database. (B) Relative transcriptional expression of cGAS in different T stages (Normal N = 68, Ta or T1 N = 126, and T2 or more N = 62) in Lee bladder cohort. (C) Western blotting of subcellular distribution of cGAS, Rad51 GAPDH, and Histone H3 proteins. GAPDH was used as cytosolic control protein, and histone H3 was used as nuclear control proteins. T24 and TCCSUP cell treated with 2 μg/mL cisplatin or PBS at 0 h, 8 h, 16 h, 24 h time points. (D) Immunofluorescence of expression and distribution of Rad51 and γ-H2A.X proteins in T24 and TCCSUP cell lines treated with μg/mL cisplatin or PBS for 24 h. DDP, cisplatin. Scale bars represent 10 μm. (E) Immunofluorescence of cGAS subcellular distribution of T24, TCCSUP, MB49 and UMUC-3 cell lines. Scale bars represent 5 μm. (F) Immunofluorescence of cGAS subcellular distribution of T24 and TCCSUP cell lines treated with 2 μg/mL cisplatin or PBS. Scale bars represent 5 μm. (G) Western blotting of subcellular distribution of cGAS, Rad51, γ-H2A.X, GAPDH and Histone H3 proteins. GAPDH was used as cytosolic and nuclear soluble control protein, and Histone H3 was used as chromatin bound control proteins. T24 and TCCSUP cells were treated with 2 μg/mL cisplatin or PBS at 0 h, 8 h, 16 h, 24 h time points. DDP, cisplatin, N.S., p > 0.05, *** p < 0.001.