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. 2022 Oct 3;119(41):e2208708119. doi: 10.1073/pnas.2208708119

Fig. 1.

Fig. 1.

phyA was the main regulator of photoperiodic flowering in soybean. (A, B) The gene structure of soybean PHYA2, PHYA3 molecules (A) and PHYB1, PHYB2 (B). (C, D) The red bar indicates where the single guide RNA (sgRNA) is. CRISPR/Cas9-induced 5 + 16-bp deletion at target site for phyA3, 1-bp insertion at target site detected for phyA2 (C) and 1-bp deletion at target site for phyB1, 4-bp deletion at target site for phyB2 (D) by Sanger sequencing. Flowering time of wild-type plants (WT, W82) and homozygous mutant phyA2 phyA3 (E, F), WT, phyB1 phyB2 (G, H) mutants under LD conditions (16-h light/8-h dark) and SD (12-h light/12-h dark).