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. 2022 Oct 3;119(41):e2208708119. doi: 10.1073/pnas.2208708119

Fig. 3.

Fig. 3.

phyA2 and phyA3 are largely but not totally dependent on LUX. (A) Phenotypes of lux1 lux2 and phyA2 phyA3 lux1 lux2 mutants under LD (16-h light/8-h dark). (B) Flowering time of W82, phyA2 phyA3, lux1 lux2, phyA2 phyA3 lux1 lux2 mutants under LD conditions (16-h light/8-h dark). Different letters indicate significant differences by Student’s t test (P < 0.05). The flowering time is shown as the mean values ± SD, n ≥ 5 plants. (C) Schematic of the E1 gene and regions tested for enrichment in the ChIP assay. (D) ChIP of E1 amplicons using W82, phyA2 phyA3, and lux1 lux2 plants at Zeitgeber time 4. Native monoclonal antibody raised against phyA3 was used for ChIP assays.