Fig. 1.

Single cell interrogation of the rat uterine–placental interface. (A) Schematic showing isolation of the rat uterine–placental interface from gd 15.5 or 19.5. Uterine tissue at the site of trophoblast invasion was peeled away from placental tissue and associated decidua. (B and C) Visualization of cell clusters from gd 15.5 and 19.5, respectively, plotted using UMAP. (D and E) Dot plots for gd 15.5 and 19.5 showing marker transcripts used to identify cell clusters. Dot size represents the average percentage of cells expressing the transcript. Dot colors correspond to cell types. For transcript expression level, see SI Appendix, Fig. S2 and Dataset S1. Abbreviations: UPI, uterine–placental interface; JZ, junctional zone; LZ, labyrinth zone.