Fig 3. BC2059 inhibits the binding of β-catenin to TBL-1 resulting in decreased β-catenin activity.

A) TBL1 expression after co-immunoprecipitation (IP) of β-catenin. Lysates from three desmoid cell lines treated with 100 nM of BC2059 or vehicle were subjected to β-catenin immunoprecipitation by magnetic beads using a specific anti-β-catenin antibody. Whole-cell lysates (input) and immunoprecipitants were analyzed by immunoblotting with an anti-TBL1 antibody. B) Cytoplasmic and nuclear expression of β-catenin in 3 DT cell lines after treatment with BC2059 for 96h. C) mRNA levels of AXIN2 measured by quantitative real-time PCR after 48h treatment with BC2059. Error bars represent SD from 3 independent experiments. (*P < 0.05; **P < 0.001).