Fig. 2.
ACBP/DBI neutralization activates autophagy/mitophagy flux and reduces cardiac damage in mice. (A and B) α-DBI or IgG (2.5 µg/g, 6 and 2 h before sacrifice) and leupeptin (30 mg/kg, 2 h before sacrifice) were injected i.p. in C57BL/6 mice. Immunoblot of LC3B (A) and densitometric quantification (B) from heart extracts (n = 6 mice per group). (C and D) Mito-Keima transgenic mice were injected twice with IgG or α-DBI i.p. 6 and 2 h before sacrifice (2.5 µg/g). Representative confocal microscopy images (C) and quantification of signals (D) (n = 3 mice per group). (E–G) Wt (Atg7fl/fl, αMHC Cre−) and homozygous Atg7−/− (Atg7fl/fl, αMHC Cre+) mice were subjected to 3 h of ischemia (E). Mice were injected with IgG or α-DBI i.p. 4 h before ischemia and just before ischemia. Representative images of left ventricular myocardial sections after Alcian blue and 2,3,5-triphenyltetrazolium chloride staining (F) and quantification of the infarction size versus area at risk of myocardial damage (G) (n = 4–6 mice per group). Results are displayed as means ± SEM. For statistical analyses, P values were calculated by ANOVA test (B and G) or two-tailed unpaired Student’s t test (D). AAR, area at risk.