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WB of E. coli lysate expressing recombinant GRA7 or recombinant GRA7 fragments. Lanes 2, 4, 6, 8, and 10, lysates of noninduced cultures; lanes 3, 5, 7, 9, and 11, lysates of induced cultures. Lane 1, protein marker; lanes 2 and 3, leader peptider only; lanes 4 and 5, GRA7; lanes 6 and 7, GRA7BS; lanes 8 and 9, GRA7BN; lanes 10 and 11, GRA7BA. (A) Reaction with anti-mTNF MAb; (B) reaction with MAb BATO 214.
