. 2022 Sep 26;12(19):3350. doi: 10.3390/nano12193350

Table 1.

Physico-chemical characteristics of the polymer precursors.

Polymer Precursor	Structure	Functional Group	Functional Groups (mol. %) ^a	M_w (g/mol) ^b	Ð ^b	D_H (nm) ^c
P0a P0b	poly(HPMA-co- MA-AP-TT)	TT	(a) 1.9 (b) 10.2	18,500 39,200	1.03 1.04	5.9 ± 0.7
P0a P0b	poly(HPMA-co- MA-AP-TT)	TT	(a) 1.9 (b) 10.2	18,500 39,200	1.03 1.04	7.4 ± 0.4
P1	poly(HPMA-co- MA-AP-COOH)	COOH	1.9	18,700	1.05	5.3 ± 0.2
P2	poly(HPMA-co-APMA)	NH₂	5.1	24,100	1.04	4.9 ± 0.1
P3	poly(HPMA-co- MA-Acap-cholesterol)	cholesterol	2.3	26,400	1.06	39.9 ± 1.1
P4	poly(HPMA-co-APMA-co- MA-AH-cholesterol)	cholesterol + NH₂	2.5 cholesterol 2.3 NH₂	24,600	1.07	26.8 ± 0.7
P5	poly(HPMA-co- MA-AP-DBCO)	DBCO	8	40,000	1.06	11.5 ± 0.8

^a Contents of TT, DBCO, and NH₂ groups were evaluated by UV–VIS spectrophotometry; the content of cholesterol was determined using NMR Spectroscopy. ^b The weight-average molecular weight (M_w) and dispersity (Ð) were determined using SEC with RI and MALS detection. The analysis was performed using a TSK 3000 SWXL column with methanol/0.3 M sodium acetate buffer, pH 6.5 (4/1, v/v), as a mobile phase. ^c The mean hydrodynamic diameter (D_H) was obtained by DLS with intensity of scattered light detected at angle θ = 173° in PBS.