Surface exposure of HSV epitope gD(272–279) (A) and TGEV epitope S(379–388) (B) on bacteria expressing hybrid fimbriae, by native dot blot immunoassay. (A) Row 1, HSV epitope expressed in the cell but not on fimbriae (negative control); row 2, no epitope in FasA; row 3, epitope in position 29 of FasA; row 4, epitope in position 97 of FasA; row 5, epitope in position 97(−2) of FasA; row 6, epitope in position 192 of FasA. Column a, 2.5 × 107 bacteria/dot; column b, 6.3 × 106 bacteria/dot. (B) Row 1, no TGEV epitope in FasA; row 2, TGEV epitope in position 26 of FasA; row 3, epitope in position 29 of FasA; row 4, epitope in position 97 of FasA; row 5, epitope in position 97(−2) of FasA; row 6, epitope in position 192 of FasA. Column a, 6.3 × 106 bacteria/dot; column b, 1.6 × 106 bacteria/dot.