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mRNA expression and endosomal release. Expression of luciferase protein (A) 30 h and 50 h after transfection with luciferase mRNA loaded on differently charged gelatin nanoparticles or commercial lipoplexes (LMM) and (B) 30 h after transfection with the stimulation of endosomal escape by the addition of chloroquine at different time points (n = 4). **** indicates statistically significant differences compared to the untransfected control (no mRNA) with p < 0.0001.
