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. 2022 Oct 14;17(10):e0275874. doi: 10.1371/journal.pone.0275874

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© 2022 Kumar et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — LDH activity and protein expression of AOEs and cytokine IL-1β (A, B). (A) LDH activity was performed in U1 cells treated with different concentrations of BaP (daily treatment up to 72 h). (B) Representative Western blots of AOEs (SOD1 and CAT) and IL-1β in U1 cells (Western blot at 72 h) treated with different concentrations of BaP (daily treatment up to day 3). Untreated controls were considered as 100% in the densitometry analysis. (C) Densitometry analysis. (D) mRNA expression was analyzed in U1 cells (at 24, 48, and 72 h) treated with 1 μM concentration of BaP (daily treatment). Statistical analyses were carried out by using one-way ANOVA (multiple comparison) and Student’s t-test (two groups). Results are expressed as means ± S.E.M of n = 3–4 experiments.