TABLE 1.
Differences due to reagent sources in cytokine and soluble activation marker levels in plasma from HIV-positive subjects
| Analyte (units of concn) | Commercial kit (type) from: | n | Mean level ± SD | P value (paired t test) | Pearson’s correlation coefficient |
|---|---|---|---|---|---|
| β2M (mg/liter) | Pharmacia (EIA) | 14 | 3.00 ± 1.25 | 0.0004a | 0.66a |
| Abbott (MEIA) | 1.91 ± 0.70 | 0.0099b | 0.58b | ||
| ORGenTec (EIA) | 2.25 ± 1.00 | 0.0085c | 0.91c | ||
| Neopterin (nmol/liter) | ICN (EIA) | 17 | 28.1 ± 13.2 | 0.00001 | 0.95 |
| BRAHMS (RIA) | 22.2 ± 10.9 | ||||
| sIL-2R (pg/ml) | Endogen (EIA) | 20 | 8,708 ± 6,118 | 0.00002 | 0.58 |
| Immunotech (EIA) | 2,205 ± 1,295 | ||||
| sTNF-RII (ng/ml) | HyCult (EIA) | 42 | 4.59 ± 1.59 | 0.00001 | 0.96 |
| Genzyme (EIA) | 3.82 ± 1.97 | ||||
| TNF-α (pg/ml) | Innogenetics (EIA) | 35 | 335.8 ± 435 | 0.1032 | 0.91 |
| Medgenix (EIA) | 398.0 ± 638 | ||||
| IFN-γ (units/liter) | Immunotech (EIA) | 12 | 532 ± 598 | 0.0166 | 0.98 |
| T-Cell Diagnostics (EIA) | 452 ± 532 | ||||
| IL-10 (pg/ml) | Immunotech (EIA) | 23 | 49.8 ± 30.1 | 0.00004 | 0.03 |
| Endogen (EIA) | 12.6 ± 9.3 |
a
Pharmacia β2M versus Abbott β2M.
b
Pharmacia β2M versus ORGentec β2M.
c
ORGentec β2M versus Abbott β2M.