Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Protein G magnetic beads conjugated with antibodies | Dynabeads, Life Technology | Y13 clone antibody specific to murine H-2Kb TP25.99.8.4 clone antibody specific to human HLA-A,B,C |
| FITC-conjugated H2-Kb antibody | Biologend | clone AF6–88.5 |
| APC-conjugated anti-H-2Db antibody | Biolegend | clone KH95 |
| PerCP/Cy5.5-conjugated anti-HLA-A2 antibody | BD Biosciences | clone BB7.2 |
| immunoproteasome detection kit | UPBIO | J4160 |
| classical proteasome detection kit | UPBIO | J4120 |
| Anti-nitrotyrosine antibody | Sigma | N0409 |
| Ani-PD-1 | BioXcell | clone RMP1–14 |
| Anti-CTLA-4 | BioXcell | clone 9D9 |
| Anti-DDX6 | ThermoFisher | PA555012 |
| Anti-PRICKLE1 | ThermoFisher | 22589–1-AP |
| Anti-ELF3 | ThermoFisher | PA589261 |
| Anti-LDHB | ThermoFisher | PA596736 |
| Anti-IFT25 | ThermoFisher | 15732–1-AP |
| Anti-PICK1 | ThermoFisher | PA1073 |
| Anti-STT3A | ThermoFisher | 12034–1-AP |
| Anti-RPS15A | ThermoFisher | PA5103532 |
| Anti-beta Actin (BA3R) | ThermoFisher | MA515739 |
| 5-(and 6)-Carboxyfluorescein diacetate succinimidyl ester Kit, CFDA SE Kit | Biolegend | 423801 |
| Anti-mouse IFN-γ mAb (AN18) | Mabtech | 3321–3-250 |
| Chemicals, peptides, and recombinant proteins | ||
| Peptides used in the study are listed in Figure S1 | GenScript | N/A |
| Tumor Dissociation Kit, mouse | Miltenyi | 130–096-730 |
| CD8a (Ly-2) MicroBeads, mouse | Miltenyi | 130–117-044 |
| Omaveloxolone (RTA-408) | Reata Pharmaceutical | N/A |
| Nitroaspirin (NCX-4016) | Sigma | SML1669 |
| Hoechst 33342 dye | ThermoFisher | H3570 |
| N-Nitro-L-arginine methylester (L-NAME) | Sellechem | S2877 |
| Deposited data | ||
| The mass spectrometry proteomics data | MassIVE (http://massive.ucsd.edu) | MSV000087990 |
| The mass spectrometry proteomics data | ProteomeXchange (http://www.proteomexchange.org) | PXD027956 |
| Experimental models: Cell lines | ||
| UACC-903 | Originally from University of Arizona Cell Collection Provided by Dr. M. Herlyn, Wistar Institute | RRID:CVCL_4052 |
| EG7 | ATCC | CRL-2113 |
| B16F10 | ATCC | CRL-6475 |
| B16F10-iNOS | This manuscript | This manuscript |
| T2 cells | ATCC | CRL-1992 |
| RMA-S cells | Gift from Dr. Eisenlohr | RRID: CVCL_2180 |
| EL-4 | ATCC | TIB-39 |
| LLC | ATCC | CRL-1642 |
| CT26 | ATCC | CRL-2638 |
| Experimental models: Organisms/strains | ||
| PMEL transgenic mice B6.Cg-Thy1a/Cy Tg(TcraTcrb)8Rest/J | Jackson Lab | RRID:IMSR_JAX:005023 |
| BALB/c mice | Charles River | |
| C57BL/6 mice | Charles River | |
| C57BL/6 mice | Envigo | |
| Oligonucleotides See Table S9 | ||
| Biological Samples | ||
| Tissue samples | University of Lausanne | Cancer Immun. 2004 May 19;4:4. PMID: 15149168 |
| Biological Samples | ||
| Tissue samples | University of Pennsylvania | Nature. 2017 May 4;545(7652):60–65. 10.1038/nature22079 |
| Software and algorithms | ||
| GraphPad Prizm | Dotmatics | 8.4.3 |