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. 2022 Oct 7;11:e80760. doi: 10.7554/eLife.80760

Table 1. Primers for plasmid generation, luciferase assays, qPCR, and ChIP-qPCR.

Primers for plasmid generation
zf zbtb14 Forward 5'-CCGGAATTCTCCGAAACTGTGAAGTATGTG-3'
Reverse 5'-CCGCTCGAG TTATGAGCAGGCGATGGACTC-3'
zf zbtb14K40R Forward 5'-GTGGAGGATGTGAGGTTCAGGGCGCAT-3'
Reverse 5'ATGCGCCCTGAACCTCACATCCTCCAC-3'
zf zbtb14K259R Forward 5'-GCCACCGCTGACATGAGGTTTGAGTATCTGCTG-3'
Reverse 5'-CAGCAGATACTCAAACCTCATGTCAGCGGTGGC-3'
tol2-mpeg1.1-pu.1 DBD Forward 5'-CGGGGTACCATGAATTCGCTTGTATCAGTTCCTGC-3'
Reverse 5'-CCGCAATTGTTAGAGAACCTCTCCACTGAACTGG-3'
hs ZBTB14 Forward 5'-CCGGAATTCATGGAGTTTTTCATCAGTATG-3'
Reverse 5'-CCGCTCGAGCTAGCTACAGGCTATCGTCTC-3'
hs ZBTB14S8F Forward 5'-GGAGACGTCAAAGTAAGAAAGGAGAGAGACCCTTGA-3'
Reverse 5'-TCAAGGGTCTCTCTCCTTTCTTACTTTGACGTCTCC-3'
hs ZBTB14S8A Forward 5'-TTCATCAGTATGGCTGAAACCATTAAA-3'
Reverse 5'-TTTAATGGTTTCAGCCATACTGATGAA-3'
hs ZBTB14S8D Forward 5'-TTCATCAGTATGGATGAAACCATTAAA-3'
Reverse 5'-TTTAATGGTTTCATCCATACTGATGAA-3'
hs ZBTB14S8W Forward 5'-TTTTTCATCAGTATGTGGGAAACCATTAAATAT-3'
Reverse 5'-ATATTTAATGGTTTCCCACATACTGATGAAAAA-3'
hs ZBTB14S8Y Forward 5'-CCGGAATTCATGGAGTTTTTCATCAGTATGTAT-3'
Reverse 5'-CCGCTCGAGCTAGCTACAGGCTATCGTCTCCAG-3'
Primers for luciferase assays
zf zbtb14-Promoter Forward 5'-CGGGGTACCCATCAGTTGTATCTTAGGTACAG-3'
Reverse 5'-CCGCTCGAGTGGACTCCTCATGTTTGCTCT-3'
zf pu.1-Promoter Forward 5'-CGGGGTACCACTAGTACACCTAAATTTATG-3'
Reverse 5'-CCGCTCGAGATTTGGCAGACCAACAACTGC-3'
Primers for quantitative PCR
zf β-actin Forward 5’-TGCTGTTTTCCCCTCCATTG-3’
Reverse 5’-TTCTGTCCCATGCCAACCA-3’
zf zbtb14 Forward 5’-CTCGTGTGTTCGGCAGTAATTG-3’
Reverse 5’-CTGGAGCGAAATCCTTACTGG-3’
zf pu.1 Forward 5’-TCCCAGCAGTCGTAGTCCTC-3’
Reverse 5’-CCATTTCGCAGAAGGTCAA-3’
zf c-myc Forward 5’-CAATTCTGGAACGGCATTCG-3’
Reverse 5’-GAAGTAGAAGTAGGGCTGGATG –3’
hs β-ACTIN Forward 5’-CCAACCGCGAGAAGATGA-3’
Reverse 5’-CCAGAGGCGTACAGGGATAG-3’
hs ZBTB14 Forward 5’-CAGGATATGGGTCTGCAGGA-3’
Reverse 5’-TCTTAATGCCTTGAACGCCA-3’
Primers for ChIP-qPCR
Negative control primer Forward 5’-GCTGAAATTTTGTAGTCTGTC-3’
Reverse 5’-ATAAGATTTTAGTCATCAAAC-3’
Positive primer Forward 5’-ACTAGTACACCTAAATTTATG-3’
Reverse 5’-GACAGACTACAAAATTTCAGC-3’