zf zbtb14
|
Forward 5'-CCGGAATTCTCCGAAACTGTGAAGTATGTG-3' |
|
Reverse 5'-CCGCTCGAG TTATGAGCAGGCGATGGACTC-3' |
zf zbtb14K40R
|
Forward 5'-GTGGAGGATGTGAGGTTCAGGGCGCAT-3' |
|
Reverse 5'ATGCGCCCTGAACCTCACATCCTCCAC-3' |
zf zbtb14K259R
|
Forward 5'-GCCACCGCTGACATGAGGTTTGAGTATCTGCTG-3' |
|
Reverse 5'-CAGCAGATACTCAAACCTCATGTCAGCGGTGGC-3' |
tol2-mpeg1.1-pu.1 DBD
|
Forward 5'-CGGGGTACCATGAATTCGCTTGTATCAGTTCCTGC-3' |
|
Reverse 5'-CCGCAATTGTTAGAGAACCTCTCCACTGAACTGG-3' |
hs ZBTB14
|
Forward 5'-CCGGAATTCATGGAGTTTTTCATCAGTATG-3' |
|
Reverse 5'-CCGCTCGAGCTAGCTACAGGCTATCGTCTC-3' |
hs ZBTB14S8F
|
Forward 5'-GGAGACGTCAAAGTAAGAAAGGAGAGAGACCCTTGA-3' |
|
Reverse 5'-TCAAGGGTCTCTCTCCTTTCTTACTTTGACGTCTCC-3' |
hs ZBTB14S8A
|
Forward 5'-TTCATCAGTATGGCTGAAACCATTAAA-3' |
|
Reverse 5'-TTTAATGGTTTCAGCCATACTGATGAA-3' |
hs ZBTB14S8D
|
Forward 5'-TTCATCAGTATGGATGAAACCATTAAA-3' |
|
Reverse 5'-TTTAATGGTTTCATCCATACTGATGAA-3' |
hs ZBTB14S8W
|
Forward 5'-TTTTTCATCAGTATGTGGGAAACCATTAAATAT-3' |
|
Reverse 5'-ATATTTAATGGTTTCCCACATACTGATGAAAAA-3' |
hs ZBTB14S8Y
|
Forward 5'-CCGGAATTCATGGAGTTTTTCATCAGTATGTAT-3' |
|
Reverse 5'-CCGCTCGAGCTAGCTACAGGCTATCGTCTCCAG-3' |
|
Primers for luciferase assays
|
zf zbtb14-Promoter
|
Forward 5'-CGGGGTACCCATCAGTTGTATCTTAGGTACAG-3' |
|
Reverse 5'-CCGCTCGAGTGGACTCCTCATGTTTGCTCT-3' |
zf pu.1-Promoter
|
Forward 5'-CGGGGTACCACTAGTACACCTAAATTTATG-3' |
|
Reverse 5'-CCGCTCGAGATTTGGCAGACCAACAACTGC-3' |
|
Primers for quantitative PCR
|
zf β-actin
|
Forward 5’-TGCTGTTTTCCCCTCCATTG-3’ |
|
Reverse 5’-TTCTGTCCCATGCCAACCA-3’ |
zf zbtb14
|
Forward 5’-CTCGTGTGTTCGGCAGTAATTG-3’ |
|
Reverse 5’-CTGGAGCGAAATCCTTACTGG-3’ |
zf pu.1
|
Forward 5’-TCCCAGCAGTCGTAGTCCTC-3’ |
|
Reverse 5’-CCATTTCGCAGAAGGTCAA-3’ |
zf c-myc
|
Forward 5’-CAATTCTGGAACGGCATTCG-3’ |
|
Reverse 5’-GAAGTAGAAGTAGGGCTGGATG –3’ |
hs β-ACTIN
|
Forward 5’-CCAACCGCGAGAAGATGA-3’ |
|
Reverse 5’-CCAGAGGCGTACAGGGATAG-3’ |
hs ZBTB14
|
Forward 5’-CAGGATATGGGTCTGCAGGA-3’ |
|
Reverse 5’-TCTTAATGCCTTGAACGCCA-3’ |
|
Primers for ChIP-qPCR
|
Negative control primer |
Forward 5’-GCTGAAATTTTGTAGTCTGTC-3’ |
|
Reverse 5’-ATAAGATTTTAGTCATCAAAC-3’ |
Positive primer |
Forward 5’-ACTAGTACACCTAAATTTATG-3’ |
|
Reverse 5’-GACAGACTACAAAATTTCAGC-3’ |