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. 2022 Oct 6;11:e80282. doi: 10.7554/eLife.80282

Figure 3. Phospholipids are elevated in whole fly during extended high-sugar diet (HSD) feeding.

Average concentrations of phosphatidylcholine (PC), phosphatidylethanolamine (PE), lysophosphatidylcholine (LPC), and lysophosphatidylethanolamine (LPE) in flies subjected to normal food (NF) (green) or an HSD (red) for 14 days. Lipidomics was performed using a targeted quantitative lipidyzer (Sciex 5500 Lipidyzer). Ten independent biological replicates were used for each diet and each day, with n = 10 flies composing one biological replicate. Two-way ANOVA and Sidak post-test correction. Asterisks indicate significant changes with p-value<0.05, p-value<0.005, and p-value<0.0005. Error bars = standard deviation.

Figure 3—source data 1. Lipidomics data for lipid classes of normal food (NF) and high-sugar diet (HSD)-fed flies on day 7 presented in Figure 3 and Figure 3—figure supplement 1.
elife-80282-fig3-data1.xlsx (369.6KB, xlsx)
Figure 3—source data 2. Lipidomics data for lipid classes of normal food (NF) and high-sugar diet (HSD)-fed flies on day 14 presented in Figure 3 and Figure 3—figure supplement 1.
elife-80282-fig3-data2.xlsx (412.3KB, xlsx)

Figure 3.

Figure 3—figure supplement 1. High-sugar diet (HSD) alters the lipidome and increases the concentration of phosphatidylethanolamine (PE) and phosphatidylcholine (PC) double bond species.

Figure 3—figure supplement 1.

(A) Changes in triacylglyceride (TAG), diacylglyceride (DAG), free fatty acid (FFA), Cholesterol Ester (CE), and Sphingomyelin (SM) concentration after 7 and 14 days of normal food (NF) and HSD treatment. (B) Average concentration of double bonds for PE (C) and PC (D) at 7 and 14 days of NF or HSD treatment. Two-way ANOVA with Holm–Sidak correction. Asterisks indicate significant changes with p-value<0.05, p-value <0.005, and p-value<0.0005. Error bars = standard deviation.