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. 2022 Oct 6;11:e80282. doi: 10.7554/eLife.80282

Figure 4. High-sugar diet (HSD) and knockdown of Pect lead to decreased ApoII levels in the brain.

(A–C, left) Representative confocal images of ApoII (red) levels in the insulin-producing cells (IPCs) (blue) of (A) normal food (NF) and HSD-fed flies on day 14, (B) flies with a fat-specific knockdown of Luc, eas, and Pect, and (C) flies with a fat-specific overexpression of Luc and Pect. (A–C, right) Mean number of ApoII puncta and % area covered by ApoII puncta in the IPCs of (A) NF and HSD-fed flies on day 14, (B) flies with a fat-specific knockdown of Luc, eas, and Pect, and (C) flies with fat-specific overexpression of Luc and Pect. N = each square represents a single fly. All data were collected from a single experiment. Unpaired t-test with Welch’s correction. Asterisks indicate significant changes with p-value<0.05, p-value<0.005, and p-value<0.0005. Error bars = standard deviation.

Figure 4—source data 1. ApoII accumulation in the insulin-producing cells (IPCs) of normal food (NF) and high-sugar diet (HSD)-fed w1118 flies.
Figure 4—source data 2. ApoII accumulation in the insulin-producing cells (IPCs) of phosphatidylethanolamine (PE) knockdown flies.
Figure 4—source data 3. ApoII accumulation in the insulin-producing cells (IPCs) of Pect overexpression flies.

Figure 4.

Figure 4—figure supplement 1. Validation of ApoII antibody.

Figure 4—figure supplement 1.

Representative confocal images of LppGal4>UAS-HA-Apolpp-myc fly fat body stained for ApoII antibody (green), HA antibody (red), and a merge of the two images. Note colocalization of HA and ApoII.