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. 2022 Oct 17;132(20):e158346. doi: 10.1172/JCI158346

Figure 5. l-kynurenine supplementation rescues the preeclampsia-like phenotype in UNx mice.

Figure 5

(A) Schematic representation of the experimental design. (B) MAP measurements in UNx mice and UNx mice treated with l-kynurenine (25 mg/L) added to drinking water (UNx+kyn) in the NP state and during pregnancy. (C) Urine albumin/creatinine ratio for UNx and UNx+kyn mice. (D) Plasma cystatin C measurements in UNx and UNx+kyn mice. (E) The eGFR was calculated for UNx and UNx+kyn mice as described in Methods. (F) Histopathological analysis of renal tissue at GD18 from 1 representative UNx mouse and 1 UNx+kyn mouse. H&E-stained images (scale bars: 50 μm) and EM analysis (scale bars: 1 μm) show a normalized glomerulus structure in the UNx+kyn mouse (arrows show preserved endothelial fenestration) compared with the UNx mouse (arrows show enlarged ECs with segmental loss of fenestration). (G) The glomerular capillary area at GD18, quantified using ImageJ as described in Methods, was decreased in UNx+kyn mice compared with UNx mice. Data are presented as the mean ± SD. n = 6 per group for all experiments. *P < 0.05 in UNx versus UNx+kyn mice, by unpaired, 2-tailed Student’s t test.