Figure 4.
NE attachment neither blocks Zfp42R gene activation nor their communication with Fat1 enhancers
(A) Hi-C from wild-type and ΔD1+2 E11.5 limb buds, with the former reproduced from Figure 1.
(B) Staining of endogenous Fat1 (WISH, left) or integrated β-globin LacZ sensors (LacZ, right) in E12.5 embryos. n = 4–10 embryos. Integration sites are indicated by lines and their NE attachment in limb by black (LAD) or gray (non-LAD) boxes. Staining is indicated in the ear (e), distal limb (dl), proximal limb (pl), mammary glands (m), and face (f). Scale bar, 1 mm.
(C) Summary of gene, enhancer, and sensor activities with LAD-status indicated.