Figure S2.

Extended Zfp42R and Fat1 scRNA-seq gene expression analysis and expanded promoter mapping, related to Figure 1

(A–C) UMAPs from re-processed scRNA-seq from whole gastrulating embryos (A), the developing placenta (B), and whole embryos during organogenesis (C) (Cao et al., 2019; Marsh and Blelloch, 2020; Pijuan-Sala et al., 2019). UMAP embedding is colored according to cell type (left), developmental stage (middle), or expression of Triml2, Zfp42 or Fat1 (right). Zfp42R genes (Triml2 and Zfp42) are expressed in the extraembryonic ectoderm and endoderm (A) and placental trophoblasts (B). Zfp42 is also expressed in the E6.5 epiblast (A). Fat1 is expressed widely in many tissues (A–C) but is absent, for example, in blood progenitors and erythroid cells (A and C).

(D) Zoom of the centromeric TAD arm, Zfp42R and Fat1 gene body with H3K4me3 and H3K36me3 ChIP-seq shown. Note that Triml1 and Triml2 are transcribed from a single shared bidirectional promoter as indicated by a single peak of H3K4me3 and broad H3K36me3 marking the transcribed gene body.