FIG. 3.

Specificity analysis of purified BIV p26 and tTM fusion proteins expressed in Sf9 cells from recombinant baculoviruses. A total of 200 ng of purified tTM (lanes 1) and p26 (lanes 2) recombinant fusion proteins was electrophoresed, transblotted to nitrocellulose membranes, and exposed to a 1:100 dilution of horse sera positive (A1) or negative (A2) for EIAV antibodies, to a 1:100 dilution of rabbit sera positive for BSV (B1) or BIV (B2) antibodies, and to a 1:50 dilution of bovine sera positive for BSV (C1), BLV (C2), or BIV (C3) antibodies. Lane M, molecular mass standards.