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. 2022 Sep 12;41(42):4709–4723. doi: 10.1038/s41388-022-02460-1

Fig. 4. SiRNA knockdown or pharmacological inhibition of ISCA2 induces cell death.

Fig. 4

A (i–iii) Effect of indicated siRNAs on total protein content in indicated cell lines (6 days post-transfection). B Media appearance of ACHN cells transfected with indicated siRNAs for 5 days. C Viable cell number determined by trypan blue exclusion in ACHN cells 5 days after transfection with indicated siRNAs. D Cell viability assay of 786-0 cells transfected with indicated siRNAs. Cells were seeded into 96-well plates 6 days after siRNA transfection, and cell viability was measured using resazurin daily for 6 days. E Effect of indicated siRNA on PS exposure in 786-0 cells 28 h after transfection. TRAIL (400 ng/ml, 4 h) is a positive control. F Effect of siRNA transfection in causing loss of membrane permeability in 786-0 cells 72 h after transfection using TRAIL (400 ng/ml, 72 h) as a positive control. Data shown are the mean ± SEM.