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. 2022 Sep 12;41(42):4709–4723. doi: 10.1038/s41388-022-02460-1

Fig. 6. VHL loss promotes sensitivity to ISCA2 inhibition.

Fig. 6

A ISCA2 levels in pVHL mutant ccRCC cells ± stable pVHL reconstitution. B Resazurin cell viability assay of 786-0 parental or 786-0 cells with pVHL reconstitution transfected with indicated siRNAs (24 h post-transfection). C, D Representative plot showing viability (resazurin) of RCC4 and RCC4 + VHL cells (C), and 786-0 and 786-0 +VHL cells (D), after treatment with #25 for 24 h. IC50 values (μM) are shown in brackets. E Western blots showing levels of endogenous (Endo) and overexpressed FLAG-ISCA2 in stable clones expressing empty vector (Vec) or ISCA2 from 786-0 and RCC10 cells (2 ISCA2 clones each). F, G Representative plots showing viability of RCC10 (F), or 786-0 (G) cells stably expressing empty vector (Vec) or ISCA2 treated with #25 for 24 h. IC50 values (μM) are shown in brackets. H Representative plots showing viability of RCC10 cells stably expressing empty vector (Vec) or ISCA2 treated with Erastin for 24 h. IC50 values (μM) are shown in brackets.