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. 2022 Oct 14;12:17292. doi: 10.1038/s41598-022-21708-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Linear discriminant analysis (LDA) score distribution of microbiome between (a) NO; non-obese (blue) and OB; obese (red), and (c) MHO; metabolically healthy obese (light blue) and MUO; metabolically unhealthy obese (orange). Alphabetical letters listed before the name of the enterobacteria represent p_; phylum, c_; class, o_; order, f_; family, and g_; genus levels. Linear discriminant analysis effect size (LEfSe) analyses of gut microbiota and Cladogram indicating statistical differences of the microbial populations between (b) NO; non-obese (blue) and OB; obese (red), and (d) MHO; metabolically healthy obese (light blue) and MUO; metabolically unhealthy obese (orange). Cladograms show the phylogenetic distribution of bacterial lineages associated with groups from kingdom, phylum, class, order, family, and genus levels are listed in kingdom from inside to outside of the cladogram. NO; non-obese (blue) (n = 81), OB; obese (red) (n = 59), MHO; metabolically healthy obese (light blue) (n = 54) and MUO; metabolically unhealthy obese (orange) (n = 21). Graphics were made using the Galaxy/Hutlab (https://huttenhower.sph.harvard.edu/galaxy/). p < 0.05, LDA score > 5.5.