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. 2022 Oct 14;12:17235. doi: 10.1038/s41598-022-22097-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluatin of infarct size and apoptosis markers. (a) Infarct size after GA treatment (n = 4). Top: representative 2,3,5-triphenyl-2H-tetrazolium chloride-stained heart slices for each treatment condition. Bottom: measured infarct size, normalized to the LV area, in isolated rat hearts at the end of reperfusion. (b) Cleaved caspase-3 protein levels after GA treatment (n = 4). Top: Western blot showing casepase-3 protein levels corrected to GAPDH. Bottom: Cleaved caspase-3 protein levels corrected to GAPDH. Ctr control, Rep reperfusion, GA 2 h Gum Arabic infusion 2 h before sacrifice, GA 2 W Gum Arabic administration for 2 weeks, GA 4 W Gum Arabic administration for 4 weeks, GA Rep. Gum Arabic infusion at reperfusion. *P < 0.001 compared to control. Full length gel was included in supplementary figure (Fig. 3S).