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. 2022 Oct 14;13:6078. doi: 10.1038/s41467-022-33458-8

Fig. 7. Cross-talk between fibrocytes and cancer cells promotes macrophage activation, migration and endothelial system in cancer cells.

Fig. 7

A FACS analysis of MERTK, CD64, CD14 and HLA-DR cell surface expression on monocytes or monocyte-derived macrophages. The control (green curve) corresponds to macrophages differentiated with human serum. B, C Expression of macrophage markers CD206, IL1-ra, TNF, and IL-1B in macrophages incubated with CM from A549 or A549 + fibrocytes co-cultures, n = 6. D Migration (n = 3 independent experiments, 3 technical replicates) and (E) proliferation (n = 3 independent experiments, 8 technical replicates) of macrophages incubated with CM from A549 or fibrocytes or A549 + fibrocyte co-cultures. E ET1, ETA and ETB mRNA expression in A549 or A549 + fibrocyte coculture, n = 6. G Endothelin levels of A549 or fibrocytes or A549 + fibrocytes CM, n = 3. BE, G P-values were determined using One-way ANOVA with Fisher’s LSD test. F P-values were determined using two-tailed unpaired t-test with Welsh’s correction. Source data are provided in the source data file.