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. 2022 Oct 14;12:17242. doi: 10.1038/s41598-022-22230-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of HYBID and TMEM2 and their tissue localization in human articular cartilage. (a) and (b) Copy numbers of HYBID and TMEM2 transcripts in control normal (NOR) and OA cartilage samples. The copy numbers were determined by quantitative real-time PCR assay with standard curves generated from reference plasmids. Symbols represent individual subjects, and horizontal lines indicate means. **, P < 0.01. (c) and (d) Immunohistochemistry for HYBID and TMEM2 in human cartilage tissue. Paraffin sections of the NOR and OA cartilage samples were stained with anti-HYBID antibody (HYBID), anti-TMEM2 antibody (TMEM2) or non-immune IgG (NI). Boxed areas are shown at higher magnification in the inset. Scale bars = 200 µm. Full-length images for the inset are presented in Supplementary Figure S1b.