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. 2022 Oct 14;12:17242. doi: 10.1038/s41598-022-22230-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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HYBID-dependent HA-degrading activity in OA chondrocytes and increased HA-degrading activity in IL-6-treated OA chondrocytes. (a) Abrogation of HA-degrading activity by siRNA-mediated knockdown HYBID expression. OA chondrocytes transfected with two-different siRNAs for HYBID (HYBID-1 and HYBID-2) or non-silencing RNA (Control) were cultured with HMW-HA (10 μg/ml FA-HA H1) for 48 h, and HA fragments in the culture media were analyzed by size-exclusion chromatography. The protein expression of HYBID and GAPDH (a loading control) in the transfected cells was examined by immunoblotting. (b) No inhibition of HA-degrading activity by siRNA-mediated knockdown for TMEM2 expression. OA chondrocytes transfected with two different siRNAs for TMEM2 (TMEM2-1 and TMEM2-2) or non-silencing RNA (Control) were cultured for 48 h, and HA-degrading activity was determined as described above. The protein expression of TMEM2 and GAPDH in the transfected cells was examined by immunoblotting. (c) Enhanced HA-degrading activity in OA chondrocytes by treating with IL-6. OA chondrocytes were stimulated with IL-6 (0, 10, 50 and 100 ng/ml) in the presence of sIL-6R (100 ng/ml) and cultured with FA-HA H1 for 48 h. HA-degrading activity was determined as described above. Increased HA-degrading activity appears to be saturated by treatment with 10 ng/ml IL-6 in this assay. Arrowheads indicate elution peaks of FA-HA species with 1,562, 907, 197, or 56 kDa from left to right. The uncropped full-length gels are presented in Supplementary Figure S4.