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. 2022 Oct 14;12:17242. doi: 10.1038/s41598-022-22230-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Additive effect of the HYBID expression by TNF-α and IL-6 in OA chondrocytes. (a) and (b) Effects of TNF-α and IL-6 on the mRNA and protein expression of HYBID in OA chondrocytes, respectively. OA chondrocytes at P2 were treated with TNF-α (0 or 10 ng/ml) and/or IL-6 (0, 100 ng/ml) in the presence of sIL-6R (100 ng/ml) for 24 h and 48 h, and cell lysates were subjected to quantitative real-time PCR for HYBID expression using the ΔΔCt method (a) and immunoblotting with anti-HYBID antibody, followed by densitometric analysis (b). The average HYBID:GAPDH ratio in control OA chondrocytes treated with vehicle was set at 1. Values are expressed mean ± SD (n = 3). *, P < 0.05; **, P < 0.01. The uncropped full-length gels are presented in Supplementary Figure S5.