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. 2022 Oct 14;12:17250. doi: 10.1038/s41598-022-21552-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The effects of (−)-alpha-pinene administration in the novel object approach test. Average duration of time fish spent in the (A) inner and (B) outer ‘thigmotaxis’ zone during the novel object approach test. Fish locomotion was quantified in the novel object approach test by measuring (C) swimming velocity and (D) time spent immobile. All data are presented as mean ± S.E.M. Significant differences between controls and (−)-alpha-pinene treated groups are indicated by ***(p < 0.001).