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. 2022 Oct 13;6(1):e202101305. doi: 10.26508/lsa.202101305

Figure 4. Mitofusin proline variants induce rapid perinuclear clustering of mitochondria.

Figure 4.

(A) Representative images of Flp-In TREx HEK293 expressing the indicated mitofusin variant after incubation with 0.2 μg/ml TET for 4 h. Mitochondria were labeled with MitoTracker Red CMXRos, and nuclei were labeled with NucBlue and visualized with live-cell fluorescence microscopy. Images represent maximum intensity projections. Scale bar = 5 μm. (A, B) Quantification of the mitochondrial morphology in the cell lines described in (A). Error bars represent mean ± SEM from n = 3 separate blinded experiments (>100 cells per experiment).