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. 2022 Oct 13;6(1):e202101305. doi: 10.26508/lsa.202101305

Figure 5. Perinuclear clustering of mitochondria by the proline variant is not dependent on dynein-mediated microtubule-based transport.

Figure 5.

(A) Representative images of Flp-In TREx HEK293 expression indicated the mitofusin variant after incubation with 0.2 μg/ml TET for 4 h in the presence or absence of 5 nM nocodazole. Mitochondria were labeled with MitoTracker Red CMXRos, and nuclei were labeled with NucBlue and visualized by live-cell fluorescence microscopy. Images represent maximum intensity projections. Scale bar = 5 μm. (A, B) Quantification of the mitochondrial morphology in the cell lines described in (A). Error bars represent mean ± SEM from n = 3 separate and blinded experiments (>100 cells per experiment). (C) Representative images of Flp-In TREx HEK293 with shLacZ or shDHC and expressing the indicated mitofusin variant after incubation with 0.2 μg/ml TET for 4 h. Mitochondria were labeled with MitoTracker Red CMXRos, and nuclei were labeled with NucBlue and visualized by live-cell fluorescence microscopy. Images represent maximum intensity projections. Scale bar = 5 μm. (C, D) Quantification of the mitochondrial morphology in the cell lines described in (C). Error bars represent mean ± SEM from n = 3 separate and blinded experiments (>100 cells per experiment).