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. 2022 Oct 13;6(1):e202101305. doi: 10.26508/lsa.202101305

Figure 6. Mitofusin proline variants require GTPase activity to induce perinuclear clustering of mitochondria.

Figure 6.

(A) Representative images of Mfn1-null MEFs expressing the indicated Mfn1-mNeonGreen variant. Mitochondria were labeled with MitoTracker Red CMXRos and visualized with live-cell fluorescence microscopy. Images represent maximum intensity projections. Scale bar = 5 μm. (A, B) Quantification of the mitochondrial morphology in the cell lines described in (A). Error bars represent mean ± SEM from n = 3 separate blinded experiments (>100 cells per experiment). (C) Representative images of Mfn2-null MEFs expression indicated the Mfn2-mNeonGreen variant. Mitochondria were labeled with MitoTracker Red CMXRos and visualized with live-cell fluorescence microscopy. Images represent maximum intensity projections. Scale bar = 5 μm. (C, D) Quantification of the mitochondrial morphology in the cell lines described in (C). Error bars represent mean ± SEM from at least n = 3 separate blinded experiments (>100 cells per experiment).