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. 2022 Oct 13;6(1):e202101305. doi: 10.26508/lsa.202101305

Figure S6. Flp-In TREx HEK293 treated with nocodazole or shRNA targeting dynein heavy chain (DHC).

Figure S6.

(A) Representative images of Flp-In TREx HEK293 expressing the indicated mitofusin variant after incubation with 0.2 μg/ml TET for 4 h in the presence of 0 or 5 nM nocodazole. Mitochondria were labeled with MitoTracker Red CMXRos. Cells were fixed and immunolabeled with anti-tubulin (α-Tub). Images represent maximum intensity projections. Scale bar = 5 μm. (B) Representative Western blot of α-DHC and α-tubulin in whole-protein extract from cells treated with either control shRNA (shLacZ) or shRNA against DHC (shDHC). Molecular-weight markers are indicated in kD on left. Percent knockdown represents mean knockdown of DHC n = 3 quantified by band intensities in Western blot normalized to whole-protein stain.