Figure S7. Mfn1_IM^S329P has normal GTPase activity.

(A) A kinetic plot of GTP hydrolysis of Mfn1_IM^WT and Mfn1_IM^S329P fit to an allosteric sigmoidal equation. (B) Table of kinetic parameters for Mfn1_IM^WT and Mfn1_IM^S329P: maximum velocity (V_max), Hill coefficient (h) and Michaelis–Menten constant (K_half). (C) Summary of key catalytic residues in Mfn1 with alanine substitutions and the reported impact on dimerization (G–G interface) and GTP hydrolysis. References are (1) Qi et al (2016), (2) Yan et al (2018), (3) Cao et al (2017), and (4) this work.