Figure S9. Mfn1I105A does not support mitochondrial fusion and blocks mitochondrial fusion by wild-type mitofusins.
Wild-type or Mfn1-null MEFs expressing mt-paGFP and transduced with empty vector (V) or Mfn1I105A-FLAG were imaged either 0 min or 50 min after activation of mt-paGFP with a 405-nm laser, which is time = 0. The mitochondrial network was labeled with MitoTracker Red CMXRos and visualized with live-cell fluorescence microscopy. Quantification of the diffusion of mt-paGFP in the indicated cell lines is shown. Error bars represent mean ± SEM. n = 6/7 cells over three independent experiments.